Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
Stimulation of Adipogenesis (Gly)
Total triglyceride differentiation assays are performed in a 96-well format using subcutaneous preadipocytes. Compounds are tested for their ability to increase triglyceride accumulation during the differentiation process compared to the positive control (complete differentiation medium).
Triglycerides are quantified using a coupled enzyme-colorimetric reaction and compared to a standard curve. A DMSO vehicle is a negative control, and PPARα agonists are positive controls. This service includes 10 customer-defined compound concentrations, each tested in triplicate.
The optimal sample for the service is a compound dissolved in DMSO, PBS, or culture medium.
Once we know the details of your project, we can provide you an immediate price quote (contact e-mail: info@altogenlabs.com). Please note that experimental details will help us to provide an accurate quote and timeline estimate.
see also: Teratoma formation and analysis service | mRNA, Protein, and DNA Liposome Encapsulation Service | Stimulation of Adipogenesis (Gly) | Cell Culture Decontamination Service