Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
BIOREMEDIATION
Altogen Labs extensive biotechnology and pharmacology BIOREMEDIATION include the following:
Figure 1. Degradation of Texas crude oil by natural oil-degrading bacteria. Altogen Labs strain #525712. Twenty-four (24) hour experiment (Altogen Labs).
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Bioremediation: Oil-degrading Bacteria Products and Services
In recent years, a number of commercial enterprises have sought to develop and capitalize biodegradation technologies. The existence of such companies has now become economically justifiable because of the growing costs of traditional treatment technologies that bring usurious fees in repose to high-profile ocean oil spills. The interest of commercial businesses in utilizing microorganisms to detoxify effluents is reflected in “bioremediation” having become a common buzzword in waste management circles. Companies specializing in bioremediation (or, as it was known several years ago, biodegradation technologies) are attempting to develop a viable integration of microbiology and systems engineering to profit in the oil clean-up business.
Microorganisms have also been successfully applied during the removal of the Exxon Valdez oil spill. A number of microorganisms can utilize oil as a food source, and many of them produce potent surface-active compounds that can emulsify oil in water and facilitate oil removal. Unlike chemical surfactants, microbial emulsifiers are non-toxic and biodegradable. In addition, “fertilizers“ have been utilized to increase the growth rates of the indigenous populations of bacteria that are able to degrade oil. The use of microbes for bioremediation is not limited to the detoxification of organic compounds. In many cases, selected microbes can also reduce the toxicity of heavy metals (e.g., selenium) to a much less toxic and much less soluble elemental form. Thus, the bioremediation of surface water with significant contamination by heavy metals can now be attempted.
Web addresses with links to sites related to bioremediation include the following: http://www.nal.usda.gov. As is apparent from this site, the U.S. government (i.e., the U.S. Department of Energy; DOE) has a keen interest in bioremediation [e.g., see the Natural and Accelerated Bioremediation Research (NABIR) Program at http://www.lbl.gov]. Part of this interest stems from a commitment to clean up heavily polluted sites (e.g., the Hanford site in Washington state) that once housed nuclear weapons facilities and contained large, buried metal buckets of radioactive waste that now are starting to leak. A DOE Web site on the Hanford cleanup (involving both traditional technologies and bioremediation) can be found at http://www.hanford.gov.
Bioremediation of Oil Spills: Response and Restoration
While the first responders to ocean oil spills are generally the Coast Guard and concerned corporate assets associated with the event, larger federal responses to such events fall primarily to four agencies:
National Oceanographic and Atmospheric Administration (NOAA)
Federal Emergency Management Agency (FEMA)
United States Geological Survey (USGS)
Environmental Protection Agency (EPA)
Although each of these agencies has a somewhat different focus, together they are the front line in the response to and the restoration from oil spills. Some of these groups are conducting research into microbial bioremediation and have gathered relevant data as a consequence of their participation in the Deepwater Horizon event and its ongoing clean-up effort.
Altogen Labs provide innovative life science research services, including variety of pre-clinical studies, development of stable cell lines, in vivo siRNA delivery and complete A-to-Z RNAi gene silencing services: gene targeting, siRNA synthesis, chemical modification, functional in vitro validation, siRNA encapsulation, in vivo siRNA protection and tissue targeted delivery. Our scientists apply advanced biotechnologies to develop new bioremediation products and services.