Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
Cryopreservation and Cell Banking Service (GLP-compliant)
Altogen GLP-compliant Cell Banking and Cryopreservation Service
Cryogenic storage at ultralow temperatures is recognized to provide an indefinite longevity to cells, however researchers observed noticeable variability in deterioration of cells when samples were kept at different frozen temperatures. Generally, the liquid nitrogen at -196°C is required to successfully preserve the complex biological structures and to virtually stop all biological activity.
Altogen Labs complies with regulatory guidelines to ensure that all procedures, practices and facilities are conforming. Our GLP-compliant cryopreservation and cell line banking systems provide mammalian cell bank production and services. All cell lines are certified to be free of Mycoplasma or Bacterial contamination.
Our protocols utilize suitable combinations of cryoprotectants and cooling regimes to guarantee successful cryopreservation of biological materials, cells, tissues, tissue samples, including: Stable cell lines, Cells for transfusion, Umbilical cord blood cells, Stem cells, Tumor/ histological cells.
Altogen Labs offers long-term storage of cells in a secure, controlled, and monitored environment. Cell banking work is performed within certified Class II laminar flow biosafety cabinets with HEPA filtered air. Cells are grown only in calibrated incubators with UV air and water protection. Banks are divided and stored in separate freezers to minimize risks of a freezer malfunction. The levels of the liquid nitrogen and freezer temperatures are monitored 24/7 via the monitoring system. We ensure the security of the storage facility by a fully redundant backup system.
Cryovials can be used (to expand cell population) or shipped directly to the client or to a specified third party. We ship cells on dry ice to any destination worldwide.
see also: Cryopreservation and Cell Banking Service (GLP-compliant) | Cell Banking Service: Master Cell Bank (MCB, WCB) Generation