Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
GENERATION OF STABLE CELL LINES
Altogen Labs extensive biotechnology and pharmacology GENERATION OF STABLE CELL LINES include the following:
- Generation of Stable Cell Line in 28 Days
- Stable RNAi Cell Line Development: shRNA Gene Knockdown
- Protein overexpression: Mammalian stable cell line development
Development of Stable Cell Lines
Stable cell lines expressing a protein of interest (or shRNA constructs targeting gene of interest) are important research tools and widely used in drug discovery and compound screening life science programs. Development and validation of stable cell line can be a high risk, expensive, time consuming, and laborious.
Altogen Labs uses unique experience with more than 100 cancer cell lines, highly efficient gene delivery technologies, and research expertise to produce stably expressing clonal cells in just 28 days. Company scientists bring years of cell culture experience and expertise to this service, including transfection of plasmid DNA , drug selection of clonal cells, colony pickup, functional and expression screening, generation of stable lines, validation of construct expression by real-time qRT-PCR and Western Blot analysis.
Altogen Labs offers several stable cell line generation services:
- Reporter stable cell lines (luciferase, GFP, RFP, YFP)
- Protein overexpressing cell lines
- Knockdown (RNAi) cell lines
Our scientists can transform your cell line to stably express a protein of interest, or shRNA-encoding plasmid targeting gene of interest. Clonal cell line selection and cell population expansion are performed to develop a cell line that expresses specific genes integrated into the cellular genome.
Altogen Labs provides innovative life science research services, including a variety of pre-clinical studies, development of stable cell lines, in vivo siRNA delivery, and complete A-to-Z RNAi gene silencing services including gene targeting, siRNA synthesis, chemical modification, functional in vitro validation, siRNA encapsulation, in vivo siRNA protection, and tissue-targeted delivery. Our scientists apply advanced biotechnologies to develop new products and services such as bioremediation. Altogen Labs is committed to offering the highest quality service at the lowest price for scientific research.
