Altogen Labs CRO Services:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and Toxicology Testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi Development: Targeted siRNA, qPCR Assays
- Liposome Encapsulation Services: siRNA, miRNA, DNA, Proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting: Technology Development and Evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING SERVICES
- RNA INTERFERENCE (RNAi) SERVICES
- In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi)
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi Gene Knockdown Cell Lines
- RNAi Screen Pathway Analysis: siRNA and microRNA
- RNAi Manufacturing and CRO Services
- PCR Cloning: DNA Vector Construction (shRNA)
- Quantitation sh/siRNA-induced Knockdown by qRT-PCR and WB
- Genome-wide siRNA and microRNA High-throughput Screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA Liposome Encapsulation Service
- Transient (siRNA) and Stable (shRNA) Transfection Services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION SERVICES
- MICROORGANISM ID SERVICE
GENERATION OF STABLE CELL LINES
Altogen Labs extensive biotechnology and pharmacology GENERATION OF STABLE CELL LINES include the following:
- Generation of Stable Cell Line in 28 Days
- Stable RNAi Cell Line Development: shRNA Gene Knockdown
- Protein overexpression: Mammalian stable cell line development
Development of Stable Cell Lines
Stable cell lines expressing a protein of interest (or shRNA constructs targeting gene of interest) are important research tools widely used in drug discovery and compound screening life science programs. Development and validation of stable cell lines can be high-risk, expensive, and time- and labor-consuming.
Altogen Labs has unique experience with more than 150 cancer cell lines, customizable reporter systems, highly efficient gene delivery technologies, as well as the research expertise to produce stably expressing clonal cells in just 28 days (read more about Generation of Stable Cell Lines in 28 Days Service). Company scientists bring years of cell culture experience and expertise for this service, including transfection of plasmid DNA, drug selection of clonal cells, colony pickup, functional and expression screening, generation of stable lines, validation of construct expression by real-time qRT-PCR, and Western blot analysis.
Stable cell lines are manufactured to generate constitutive overexpression or knockdown genes via the incorporation of transfected plasmid DNA or reporter gene constructs into the genome. The development of stable cell lines is an important research tool in pharmaceutical drug discovery, compound screening and gene therapy research.
Altogen Labs offers several stable cell line generation services:
- Reporter stable cell lines (luciferase, GFP, RFP, YFP)
- Protein overexpressing cell lines
- Knockdown (RNAi) cell lines
Our scientists can transform your cell line to stably express a protein of interest, or shRNA-encoding plasmid targeting gene of interest. We perform clonal cell line selection and cell population expansion to develop a cell line that expresses specific genes integrated into the cellular genome.
Altogen Labs provides innovative life science research services, including RNAi services, generation of stable cell lines, compound encapsulation services, and many other biology contract research services. Altogen Labs is committed to offering the highest quality service at the lowest price for scientific research.
For an immediate price and timeline quote, just contact us at info@altogenlabs.com or call Altogen Labs technical support: 512-433-6177. We will discuss your experimental details and any special needs, and provide an accurate quote and timeline estimate.