Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
Protein overexpression: Mammalian stable cell line development
Hybridomas (antibody-producing cell lines) and stable cell lines overexpressing proteins of interest are costly and vital assets for life science research. The generation of such protein-expressing cell lines can be a challenge that requires extensive cell culture experience and expertise.
Altogen Labs offer development of protein overexpression clonal stable cell lines based on client individual specifications. Our scientists bring years of extensive cell biology expertise and experimental experience.
Standard service offer includes:
a) Cloning: plasmid DNA encoding antibiotic-resistant gene, cloning of construct into plasmid DNA, final plasmid DNA amplification, Maxi-prep purification.
b) Stable cell line generation: transfection of plasmid DNA (20 ug) into a cell line of choice, drug selection of clonal cells, generation of stably-expressing population of cells, final validation of construct expression by real-time qRT-PCR (mRNA expression) or Western Blot (protein expression).
c) Two clonal cell lines (one cryovial each) are delivered to the customer.
Altogen Labs is GLP-compliant and provide innovative life science research services, including variety of pre-clinical studies, development of stable cell lines, in vivo siRNA delivery and complete A-to-Z RNAi gene silencing services: gene targeting, siRNA synthesis, chemical modification, functional in vitro validation, siRNA encapsulation, in vivo siRNA protection and tissue targeted delivery. Altogen Labs is committed to offer highest quality service at the lowest price for scientific research for pharmaceutical and biotechnology industry and academic institutions.
Please let us know the details of your project and we will be happy to provide you an immediate price quote (contact e-mail: info@altogenlabs.com). Experimental details will help us to provide an accurate quote and timeline estimate.
see also: Generation of Stable Cell Line in 28 Days | Stable RNAi Cell Line Development: shRNA Gene Knockdown | Protein overexpression: Mammalian stable cell line development