Altogen Labs CRO Services:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and Toxicology Testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi Development: Targeted siRNA, qPCR Assays
- Liposome Encapsulation Services: siRNA, miRNA, DNA, Proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting: Technology Development and Evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING SERVICES
- RNA INTERFERENCE (RNAi) SERVICES
- In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi)
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi Gene Knockdown Cell Lines
- RNAi Screen Pathway Analysis: siRNA and microRNA
- RNAi Manufacturing and CRO Services
- PCR Cloning: DNA Vector Construction (shRNA)
- Quantitation sh/siRNA-induced Knockdown by qRT-PCR and WB
- Genome-wide siRNA and microRNA High-throughput Screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA Liposome Encapsulation Service
- Transient (siRNA) and Stable (shRNA) Transfection Services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION SERVICES
- MICROORGANISM ID SERVICE
Generation of Stable Cell Line in 28 Days
Generation of Stably Expressing Cell Lines in 28 Days
Altogen Labs can provide development of stable cell lines in 28 days. Stable cell lines over-expressing a protein of interest (or shRNA construct targeting a gene of interest) are important research tools. However, production of stable cancer cell lines and primary cells can be very expensive, labor-intensive, and time-consuming.
Altogen Labs offers several stable cell line generation services: protein overexpressing cell lines, RNAi knockdown cell lines, and reporter stable cell lines (luciferase, GFP, RFP, YFP). Company scientists provide expert services with years of cell culture experience with more than 150 cancer cell lines (available in house). All clonal stable cell lines are developed based on individual client specifications, using highly efficient gene delivery technologies to produce stably expressing clonal cell lines in just 28 days. We can produce multiple clonal cell lines with various expression levels (low / medium / high expression).
Normally antibiotic resistance or fluorescent reporter gene markers are incorporated into the plasmid DNA construct to facilitate selection process. These selection markers can be co-expressed on the same vector or independently expressed on two separate vectors. The selection process facilitates the selection of the most efficient expressers or silencers of the gene of interest.
Standard services include transfection of plasmid DNA (10 - 20 µg), drug selection of clonal cells, colony pick up, generation of a stable line, expression and functional screening (at least 10 passages), and final validation of construct expression by qRT-PCR and/or Western blot analysis.
Western Blot analytical technique uses antibodies specific to target protein in order to quantify the expression of the protein in a sample. The genomic integration of the target gene and associated mRNA expression increase can be verified using quantitative real-time RT-PCR technique. The utilization of both techniques validate that the target gene is both integrated into the genome and functionally expressing.
For pricing and timeline estimates please contact us at info@altogenlabs.com or call Altogen Labs technical support: 512-433-6177
see also: Generation of Stable Cell Line in 28 Days | Stable RNAi Cell Line Development: shRNA Gene Knockdown | Protein overexpression: Mammalian stable cell line development