Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
Generation of Stable Cell Line in 28 Days
Generation of Stably Expressing Cell Lines in 28 Days
Altogen Labs offers development of stable cell lines in 28 days. Stable cell lines over-expressing a protein of interest (or shRNA construct targeting gene of interest) are important research tools. However, production of stable cancer cell lines and primary cells can be very laborious, expensive, and time-consuming.
Altogen Labs uses unique experience with more than 100 cancer cell lines (available in-house) and highly efficient gene delivery technologies to produce stably expressing clonal cell lines in just 28 days. Altogen Labs offers several stable cell line generation services: a) Protein overexpressing cell lines, b) Knockdown (RNAi) cell lines, c) Reporter stable cell lines (luciferase, GFP, RFP, YFP).
Altogen Labs generate clonal stable cell lines based on individual client specifications. Company scientists bring years of cell culture experience and expertise to enable this service. Standard offer include transfection of plasmid DNA (20 µg), drug selection of clonal cells, colony pick up, generation of stable line, expression and functional screening, and final validation of construct expression by real-time qRT-PCR or Western blot analysis.
see also: Generation of Stable Cell Line in 28 Days | Stable RNAi Cell Line Development: shRNA Gene Knockdown | Protein overexpression: Mammalian stable cell line development