Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
ELISA and Cell-based Assay Development
Altogen Labs possesses extensive experience in creating unique and novel approaches to immune- and cell-based methods. Platforms and approaches include Enzyme immunoassay (EIA), Bio-Plex, Kinase profiling assays, Biomarker analysis, Receptor binding, Cell signaling, Cytotoxicity, Cell Cycle assays, Cell Viability, Cell Proliferation.
Altogen Labs can develop a cell-based assay to quantify the effect of a client’s proprietary compound by quantitation of specific biomarkers, cytokines, or phosphoproteins in cell culture supernatant or whole-cell lysates.
ELISA and Cell-Based Assay Sample Analysis
Altogen Labs uses a cell-based assay to analyze customer samples with a robust ELISA-based assay. The activity of the compound of interest is tested on a panel of 16 intracellular Ser/Thr kinases and 22 Tyr kinases, using phospho-specific antibodies by the determination of Substrate-specific phosphorylation activity induced by kinase activation, IC50 concentrations of Ser/Thr kinases and Tyr kinases.
The activity of the following kinases can be profiled:
• Ser/Thr kinases: CDK2, IRAK4, GPKA, PKCβ, MAPK2, CDK4, JNK, PKB, p38α, MKK6, GS3Kβ, MAPK1, PKCα, p38β, IKK1
• Tyr Kinases: Abl, HGFR, Lck, Src, TrkC, EGF-R, IGF1-R, PDGFRα, Syk, VEGFR2, FIt-l IR, PDGFRβ, REC, Zap70, Jak2, RET, TRK
An insulin analog/insulin signaling pathway screen is available by utilizing cell lines that stably overexpress the human insulin receptor.
Once we know the details of your project, we can provide you an immediate price quote (contact e-mail: info@altogenlabs.com). Please note that experimental details will help us to provide an accurate quote and timeline estimate.
see also: Pharmacology and toxicology testing: IC50 for Tumor Cell Lines | Xenograft Animal Service: Immunocompromised NOD/SCID Mice | Therapeutic RNAi development: targeted siRNA, qPCR assays | Liposome Encapsulation services: siRNA, miRNA, DNA, proteins | ELISA and Cell-Based Assay Development (Cell Cycle, Viability) | Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen | RNAi Consulting Services: technology delevopment & evaluation