Altogen Labs CRO Services:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and Toxicology Testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi Development: Targeted siRNA, qPCR Assays
- Liposome Encapsulation Services: siRNA, miRNA, DNA, Proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting: Technology Development and Evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING SERVICES
- RNA INTERFERENCE (RNAi) SERVICES
- In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi)
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi Gene Knockdown Cell Lines
- RNAi Screen Pathway Analysis: siRNA and microRNA
- RNAi Manufacturing and CRO Services
- PCR Cloning: DNA Vector Construction (shRNA)
- Quantitation sh/siRNA-induced Knockdown by qRT-PCR and WB
- Genome-wide siRNA and microRNA High-throughput Screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA Liposome Encapsulation Service
- Transient (siRNA) and Stable (shRNA) Transfection Services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION SERVICES
- MICROORGANISM ID SERVICE
ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
ELISA and Cell-based Assay Development
Altogen Labs possesses extensive experience in creating unique and novel approaches to immune- and cell-based methods. Platforms and approaches include enzyme immunoassay (EIA), bio-plex kinase profiling assays, biomarker analysis, receptor binding, cell signaling, cytotoxicity, cell cycle assays, cell viability, and cell proliferation.
Altogen Labs can develop a cell-based assay to quantify the effect of a client’s proprietary compound by quantitation of specific biomarkers, cytokines, or phosphoproteins in cell culture supernatant or whole-cell lysates. Such technology allows researchers to apprehend the biological functions of complex systems and identify new targets of interest.
ELISA kits and cell-based assays are designed to measure protein concentrations of interest and quantify the associated biological activity of target antigens. Customizable monoclonal and polyclonal antibodies produce high affinity, specificity and sensitivity to immune- and cell- based assays to identify given target antigens.
CUSTOM SERVICES:
• Antibody Testing, Custom labeling, Biotinylation, Purification
• Monoclonal and Polyclonal Antibody Development
• ELISA Assay Development
• ELISA Platform Optimization
• ELISA Assay Format Modification
ELISA and Cell-Based Assay Sample Analysis
Altogen Labs uses a cell-based assay to analyze customer samples with a robust ELISA-based assays. Standard ELISA service include testing activity of the compound of interest on a panel of 15 intracellular Ser/Thr kinases and 17 Tyr kinases, using phospho-specific antibodies by the determination of substrate-specific phosphorylation activity induced by kinase activation.
The activity of the following kinases can be profiled:
• Ser/Thr kinases: CDK2, IRAK4, GPKA, PKCβ, MAPK2, CDK4, JNK, PKB, p38α, MKK6, GS3Kβ, MAPK1, PKCα, p38β, IKK1
• Tyr Kinases: Abl, HGFR, Lck, Src, TrkC, EGF-R, IGF1-R, PDGFRα, Syk, VEGFR2, FIt-l IR, PDGFRβ, REC, Zap70, Jak2, RET, TRK
Once we know the details of your project, we can provide you an immediate price quote (contact e-mail: info@altogenlabs.com or call Altogen Labs technical support: 512-433-6177). Please note that experimental details will help us to provide an accurate quote and timeline estimate.
see also: Pharmacology and Toxicology Testing: IC50 for Tumor Cell Lines | Xenograft Animal Service: Immunocompromised NOD/SCID Mice | Therapeutic RNAi Development: Targeted siRNA, qPCR Assays | Liposome Encapsulation Services: siRNA, miRNA, DNA, Proteins | ELISA and Cell-Based Assay Development (Cell Cycle, Viability) | Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen | RNAi Consulting: Technology Development and Evaluation