Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
Liposomal encapsulation of siRNA, shRNA, microRNA, mRNA, protein, and plasmid DNA molecules:
Liposomes are composite structures made of phospholipids that can be made to encapsulate various types of molecules within the aqueous core of the liposome. Liposomes are used for efficient delivery of cargo molecules (such as siRNA, mRNA, DNA, RNA, protein) into cells in vitro and in vivo.
Altogen Labs offer encapsulation of charged molecuIes (siRNA, shRNA, microRNA, mRNA, plasmid DNA, protein) into one of the standard liposomes (PC:Cholesterol and DSPC:Cationic Lipid: PEGylated Lipoid:Cholesterol) or using custom liposome formulation.
Sonication and liposome extrusion methods allow to generate liposomes of defined size (from 50 nm to 400 nm particles). Customer should provide at least 0.1 mg of cargo molecule siRNA for liposome encapsulation.
Altogen Labs provide innovative life science research services, including variety of pre-clinical studies, development of stable cell lines, in vivo siRNA delivery and complete A-to-Z RNAi gene silencing services: gene targeting, siRNA synthesis, chemical modification, functional in vitro validation, siRNA encapsulation, in vivo siRNA protection and tissue targeted delivery. Our scientists apply advanced biotechnologies to develop new products and services (bioremediation). Altogen Labs is committed to offer highest quality service at the lowest price for scientific research for pharmaceutical and biotechnology industry and academic institutions.
Please let us know the details of your project and we will be happy to provide you an immediate price quote (contact e-mail: info@altogenlabs.com). Experimental details will help us to provide an accurate quote and timeline estimate.
see also: Pharmacology and toxicology testing: IC50 for Tumor Cell Lines | Xenograft Animal Service: Immunocompromised NOD/SCID Mice | Therapeutic RNAi development: targeted siRNA, qPCR assays | Liposome Encapsulation services: siRNA, miRNA, DNA, proteins | ELISA and Cell-Based Assay Development (Cell Cycle, Viability) | Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen | RNAi Consulting Services: technology delevopment & evaluation