Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
BACTERIA ID 16S SEQUENCING
Altogen Labs extensive biotechnology and pharmacology BACTERIA ID 16S SEQUENCING include the following:
- Insertion/Deletion and SNP Sequencing Service
- Pyrosequencing: Detection of unknown sequence variants
- Bacterial Analysis: Pyrosequencing of 16S
- Drug Resistance
- Pyrosequencing Methylation Services
- Global Methylation Assays
Pyrosequencing technology helps answer important questions in genetic research and applied sciences. Pyrosequencing PyroMark technology provides the gold standard in quantitative DNA sequence analysis for epigenetic DNA methylation; this technology allows accurate conclusions regarding the effects of DNA methylation on cell cycle and metabolism.
Starting Template Requirements:
Template should start with less than 500 ng per reaction
Recommended: 1-10 ng of human genomic DNA
May differ slightly if studying other genomes
QC of PCR Amplicions:
It is recommended to visualize the PCR products on agarose gel prior to performing the Pyrosequencing reaction
Altogen Labs offers STANDARD and CUSTOM services:
Standard service: Use optimized reagents as recommended by the vendor (client is responsible for DNA quality and purity).
Custom service: Sample QC (agarose gel), PCR and assay optimization.
Applications:
Epigenetics:
Quantitative methylation analysis at individual and multiple contiguous CpG sites
Cancer Research:
Performance of varied assays for full characterization of disease-related genetic factors
Population Studies:
Quantification of population genetic structure, even with quick mutation rates such as viruses
Evolutionary Studies:
Phylogenetic comparisions with high sensitivity to track trait changes
Pharmacogenetics:
New marker discovery and validation
Plant Genetics:
Sequencing and quantifying homozygosity, heterozygosity, and diferent heterozygous states of poloploid organisms
Veterinary Sciences:
Detection of multiple pathogen infections and fast geneotyping for breeding programs
Forensics:
Forensic markers for identity testing, even with highly degraded DNA
What we can do with Pyrosequencing:
Quantification of alleles, even when found at low frequencies
Detection of unknown sequence variants
Built-in assay controls guaranteeing data accuracy and reliability
Fast delivery of direct and unambiguous sequence data
Because results are simply the true sequence of the DNA in a sample, the user can examine multiple mutation sites within a specified region, and even multiple variation types, all in the same run
The results provide unambiguous information since users can check the sequence surrounding the variable site to ensure that the correct DNA region has been analyzed
Please send your project details to info@altogenlabs.com, and we will be happy to provide an immediate, confidential price quote. Experimental details will help us provide accurate quotes and timeline estimates.