Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
Global Methylation Assays
PyroMark LINE-1:
LINE-1 retrotransposable elements make up about 15% of human genome. DNA methylation within the promoter region of human LINE-1 elements is important for maintaining transcriptional inactivation and for inhibiting transposition. This methylation assay amplifies a region of the LINE-1 element and serves as a marker for global methylation.
PyroMark MGMT:
The O6-Metylguanine DNA methyltransferase (MGMT) gene is located at chromosome 10(10q26). This methylation assay detects the level of methylation in position 17-39 of exon 1 of the MGMT gene.
PyroMark MTHFR:
This assay is for genotyping the C677T variant in the human methylene tetrahydrofolate reductase (MTHFR) gene. The variant replaces the nucleotide cytosine with thymine at position 677 in the MTHFR gene.
PyroMark P16:
p16 (CDKN2A) is found on chromosome 9(9p21). It is a tumor suppressor gene, frequently mutated or deleted in a wide variety of tumors. It is also inactivated by promoter hypermethylation in a number of different cancers. This methylation analysis assay detects the level of methylation in a region -24 to -11 from the transcription start site.
Service Options:
Pyrosequencing reaction only:
Customer provides PCR amplicon and Pyrosequencing primer
Altogen performs pyrosequencing reaction and produces analyzed results
PCR + Pyrosequencing reaction:
Customer provides DNA template, PCR amplicions, and Pyrosequencing primer
Altogen optimizes and performs biotinylated PCR, performs Pyrosequencing reaction, and produces analyzed results
Extraction + PCR + Pyrosequencing reaction:
Customer provides cells, tissue, biopsy, etc…
Altogen performs extraction of genomic DNA, optimizes and performs biotinylated PCR, perform Pyrosequencing reaction, and analyze results
Extraction + Bisulfite Conversion + PCR + Pyrosequencing reaction:
Customer provides cells, tissue, biopsy, etc…
Altogen performs extraction of genomic DNA, performs bisulfite conversion of genomic DNA, optimizes and performs biotinylated PCR, performs Pyrosequencing reaction, and produces analyzed results
Assay Design:
Customer provides the sequence
Altogen designs Pyrosequencing assay
see also: Insertion/Deletion and SNP Sequencing Service | Pyrosequencing: Detection of unknown sequence variants | Bacterial Analysis: Pyrosequencing of 16S | Drug Resistance | Pyrosequencing Methylation Services | Global Methylation Assays