Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
Pyrosequencing Methylation Services
Pyrosequencing technology can be used to correlate DNA methylation patterns with tumor type, gene expression, response to treatment with specific drugs and medicines, and changes in tumorigenesis patterns.
Pyrosequencing reports the ratio of cytosine to thymine at each CpG site analyzed, which reflects the proportion of methylated DNA.
Pyrosequencing assay design can be performed in either orientation and on either strand.
Contiguous CpG sites are analyzed independently and within the same run, which enables assessment of sequence-wide methylation patterns.
Service Options:
Pyrosequencing reaction only:
Customer provides PCR amplicon and Pyrosequencing primer
Altogen performs pyrosequencing reaction and produces analyzed results
PCR + Pyrosequencing reaction:
Customer provides DNA template, PCR amplicions, and Pyrosequencing primer
Altogen optimizes and performs biotinylated PCR, performs Pyrosequencing reaction, and produces analyzed results
Extraction + PCR + Pyrosequencing reaction:
Customer provides cells, tissue, biopsy, etc…
Altogen performs extraction of genomic DNA, optimizes and performs biotinylated PCR, perform Pyrosequencing reaction, and analyze results
Extraction + Bisulfite Conversion + PCR + Pyrosequencing reaction:
Customer provides cells, tissue, biopsy, etc…
Altogen performs extraction of genomic DNA, performs bisulfite conversion of genomic DNA, optimizes and performs biotinylated PCR, performs Pyrosequencing reaction, and produces analyzed results
Assay Design:
Customer provides the sequence
Altogen designs Pyrosequencing assay
see also: Insertion/Deletion and SNP Sequencing Service | Pyrosequencing: Detection of unknown sequence variants | Bacterial Analysis: Pyrosequencing of 16S | Drug Resistance | Pyrosequencing Methylation Services | Global Methylation Assays