Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
Generation of Tet-inducible RNAi gene knockdown cell lines
Generation of Tetracycline-inducible RNAi Gene Knockdown Cell Lines
Altogen Labs offers the development of inducible RNAi stable cell lines expressing shRNA constructs that target genes of interest. The generation of tetracycline-inducible RNAi stable cancer cell lines can be very complex and laborious to produce with all the necessary controls involved (e.g., efficiency of gene silencing, control for off-target effects, and promoter compatibility).
Company scientists bring years of extensive cell culture experience and expertise, including complete RNAi services (the client is only required to provide gene ID to target) the cloning of plasmid DNAs encoding antibiotic-resistant genes, the synthesis and cloning of shRNA constructs targeting genes of interest into plasmid DNAs, final plasmid DNA amplification and stable cell line generation.
Service include transfection of plasmid DNA (at least 20 µg) into a cell line of interest, drug selection of clonal cells, generation of stable cells, validation of functional shRNA construct expression, and RNAi gene silencing by real-time qRT-PCR (reduction of mRNA expression) and/or Western blot (reduction of protein expression). Two clonal cell lines (one cryovial each) are delivered to the customer.
Altogen Labs provide innovative life science research services, including variety of pre-clinical studies, development of stable cell lines, in vivo siRNA delivery and complete A-to-Z RNAi gene silencing services: gene targeting, siRNA synthesis, chemical modification, functional in vitro validation, siRNA encapsulation, in vivo siRNA protection and tissue targeted delivery.
Please let us know the details of your project, so that we can provide you an accurate quote (contact e-mail: info@altogenlabs.com) and timeline estimate.
see also: In vivo siRNA delivery and tissue-targeting | siRNA Transfection and RNAi Cell-based Library Screening | Generation of Tet-inducible RNAi gene knockdown cell lines | RNAi screen pathway analysis: siRNA and microRNA library | RNAi manufacturing and CRO services | PCR Cloning: shRNA-encoding vector construction | In Vivo Stable siRNA and microRNA - Chemical modification | Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB | Reporter gene assays and Stable RNAi cell line development | Genome-wide siRNA and microRNA high-throughput screens | Stable RNAi Cell Line Generation: Stable Gene Knockdown | siRNA liposome encapsulation service | Transient (siRNA) and stable (shRNA) transfection services