Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
Genome-wide siRNA and microRNA high-throughput screens
Altogen Labs provides genome-wide siRNA and microRNA high-throughput screens. Other RNAi services include: RNAi assay development, siRNA Transfection, RNAi cell-based library screening, siRNA/shRNA design, synthesis, in vitro validation, qRT-PCR test of target mRNA knockdown, development of RNAi cell lines, mRNA reduction in tissues, siRNA targeting, chemical modification, cell transfection optimization, siRNA and microRNA genome-wide library screening.
Inducing gene silencing (RNAi) in cell lines is powerful research approach to study gene function. However, transfection of siRNA, shRNA, or microRNA into particular cell line can be very laborious, expensive, and time-consuming. Altogen Labs offers transient and stable RNAi transfection services based on client individual specifications.
Our scientists bring years of extensive cell culture experience and expertise. Transient RNAi transfection services includes: Design, synthesis, HPLC purification, and annealing of 5 different siRNA molecules targeting gene of interest. siRNAs are tested for gene silencing potency. Two out of five siRNAs are finally selected (siRNAs are guaranteed to provide at least 80% mRNA expression knockdown). Transfection conditions are developed for a cell line(s) of choice. Validation of gene silencing (RNAi) effect by real-time qRT-PCR (mRNA expression level) or Western Blot (protein expression level).
see also: In vivo siRNA delivery and tissue-targeting | siRNA Transfection and RNAi Cell-based Library Screening | Generation of Tet-inducible RNAi gene knockdown cell lines | RNAi screen pathway analysis: siRNA and microRNA library | RNAi manufacturing and CRO services | PCR Cloning: shRNA-encoding vector construction | In Vivo Stable siRNA and microRNA - Chemical modification | Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB | Reporter gene assays and Stable RNAi cell line development | Genome-wide siRNA and microRNA high-throughput screens | Stable RNAi Cell Line Generation: Stable Gene Knockdown | siRNA liposome encapsulation service | Transient (siRNA) and stable (shRNA) transfection services