Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
Altogen Labs provide complete RNAi gene silencing services, including validation of gene silencing - performed using real-time qRT-PCR (mRNA expression level) or Western blot (protein expression level).
Altogen Labs offer siRNA design, synthesis, purification and functional testing to measure percent (%) of mRNA knockdown using HeLa cell line (or cell line of choice - 70 cell lines available).
Service includes: Design, synthesis, HPLC purification, and annealing of 5 different siRNA molecules targeting gene of interest. siRNAs are tested for gene silencing potency. Two out of five siRNAs are finally selected (siRNAs are guaranteed to provide at least 80% mRNA expression knockdown).
Validation of gene silencing (RNAi) effect performed by real-time qRT-PCR (mRNA expression) or Western Blot (protein expression). Client provides the gene name and accession number of the target gene and we’ll do the rest.
Altogen Labs Gene Knockdown by shRNAs
Another option for client is to provide gene name and accession number of the target gene(s), mail the cells and primary antibodies for Western blotting analysis to Altogen Labs, and the lab team does the rest. Altogen’s shRNA knockdown service includes design, construction, and confirmation of the 3'-5' sequence the shRNA vectors for every target gene. Design and synthesis of the SYBR-labeled qPCR primers for quantitation of target gene expression, shRNA transfection into the cell line supplied by the customer, and targeted gene expression analysis by qPCR at 24, 48, and 72 hr post-transfection and Western blot at 48 and 72 hr after transfection are performed by company scientists. Altogen Labs guarantees 80% or more inhibition of target gene expression. Transfection controls includes the negative vector control with scrambled sequences (21 bp) and a GFP protein vector control for assessment of transfection efficiency.
see also: In vivo siRNA delivery and tissue-targeting | siRNA Transfection and RNAi Cell-based Library Screening | Generation of Tet-inducible RNAi gene knockdown cell lines | RNAi screen pathway analysis: siRNA and microRNA library | RNAi manufacturing and CRO services | PCR Cloning: shRNA-encoding vector construction | In Vivo Stable siRNA and microRNA - Chemical modification | Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB | Reporter gene assays and Stable RNAi cell line development | Genome-wide siRNA and microRNA high-throughput screens | Stable RNAi Cell Line Generation: Stable Gene Knockdown | siRNA liposome encapsulation service | Transient (siRNA) and stable (shRNA) transfection services