Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
Reporter gene assays and Stable RNAi cell line development
Altogen Labs provides RNAi reporter assay and RNAi cell line development services.
RNAi stable cell lines expressing shRNA construct (to target gene of interest in long-term experiments ) are important gene expresion research tool. However, generation of RNAi stably-expressing cancer cell lines can be very laborious, expensive, and time-consuming. Altogen Labs offer generation of clonal stable knockdown cell lines based on client individual specifications. Our scientists bring years of extensive cell culture experience and expertise. Service includes:
a) Cloning: plasmid DNA encoding antibiotic-resistant gene, synthesis and cloning of shRNA construct targeting gene of interest into plasmid DNA, final plasmid DNA amplification, Maxi-prep purification.
b) Stable cell line generation: transfection of plasmid DNA (at least 20 ug should be provided by client) into a cell line of choice, drug selection of clonal cells, generation of stably-expressing population of cells, validation of shRNA construct expression and RNAi gene silencing by real-time qRT-PCR (mRNA expression) or Western Blot (protein expression).
Another option is generation of Tetracycline inducible RNAi stably-expressing cell lines.
see also: In vivo siRNA delivery and tissue-targeting | siRNA Transfection and RNAi Cell-based Library Screening | Generation of Tet-inducible RNAi gene knockdown cell lines | RNAi screen pathway analysis: siRNA and microRNA library | RNAi manufacturing and CRO services | PCR Cloning: shRNA-encoding vector construction | In Vivo Stable siRNA and microRNA - Chemical modification | Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB | Reporter gene assays and Stable RNAi cell line development | Genome-wide siRNA and microRNA high-throughput screens | Stable RNAi Cell Line Generation: Stable Gene Knockdown | siRNA liposome encapsulation service | Transient (siRNA) and stable (shRNA) transfection services