Reporter gene assays and Stable RNAi cell line development

Altogen Labs provides RNAi reporter assay and RNAi cell line development services. 

RNAi stable cell lines expressing shRNA construct (to target gene of interest in long-term experiments ) are important gene expresion research tool. However, generation of RNAi stably-expressing cancer cell lines can be very laborious, expensive, and time-consuming. Altogen Labs offer generation of clonal stable knockdown cell lines based on client individual specifications. Our scientists bring years of extensive cell culture experience and expertise. Service includes:

a) Cloning: plasmid DNA encoding antibiotic-resistant gene, synthesis and cloning of shRNA construct targeting gene of interest into plasmid DNA, final plasmid DNA amplification, Maxi-prep purification.

b) Stable cell line generation: transfection of plasmid DNA (at least 20 ug should be provided by client) into a cell line of choice, drug selection of clonal cells, generation of stably-expressing population of cells, validation of shRNA construct expression and RNAi gene silencing by real-time qRT-PCR (mRNA expression) or Western Blot (protein expression).

Another option is generation of Tetracycline inducible RNAi stably-expressing cell lines.

see also: In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi) | siRNA Transfection and RNAi Cell-based Library Screening | Generation of Tet-inducible RNAi Gene Knockdown Cell Lines | RNAi Screen Pathway Analysis: siRNA and microRNA | RNAi Manufacturing and CRO Services | PCR Cloning: DNA Vector Construction (shRNA) | Quantitation sh/siRNA-induced Knockdown by qRT-PCR and WB | Genome-wide siRNA and microRNA High-throughput Screens | Stable RNAi Cell Line Generation: Stable Gene Knockdown  | siRNA Liposome Encapsulation Service | Transient (siRNA) and Stable (shRNA) Transfection Services