Stable RNAi Cell Line Generation: Stable Gene Knockdown

Altogen Labs provides generation of stable RNAi cell lines expressing shRNAs targeting genes of interest. RNAi stable cell lines expressing shRNA constructs targeting genes of interest are important gene expression research tools. However, the generation of RNAi stable cancer cell lines and primary cells can be very laborious, expensive, and time-consuming to produce.

Altogen Labs offers the generation of stable knockdown (RNAi) cell lines based on individual client requirements and project specifications. Validation of gene silencing is performed using real-time qRT-PCR (mRNA expression level) or Western blot (protein expression level). Company scientists bring years of extensive cell culture experience and cell line development expertise.

Service include:

a) cloning (plasmid DNAs encoding antibiotic-resistant genes, synthesis and cloning of shRNA constructs targeting genes of interest into plasmid DNAs, final plasmid DNA amplification, and Maxi-prep purification) and

b) stable cell line generation (transfection of plasmid DNA into the cell line of choice, drug selection, generation of stable cells, validation of shRNA construct expression, and RNAi gene silencing by real-time qRT-PCR or Western blot).

see also: In vivo siRNA delivery and tissue-targeting | siRNA Transfection and RNAi Cell-based Library Screening | Generation of Tet-inducible RNAi gene knockdown cell lines | RNAi screen pathway analysis: siRNA and microRNA library | RNAi manufacturing and CRO services | PCR Cloning: shRNA-encoding vector construction | In Vivo Stable siRNA and microRNA - Chemical modification | Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB | Reporter gene assays and Stable RNAi cell line development | Genome-wide siRNA and microRNA high-throughput screens | Stable RNAi Cell Line Generation: Stable Gene Knockdown  | siRNA liposome encapsulation service | Transient (siRNA) and stable (shRNA) transfection services