Altogen Labs CRO Services:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and Toxicology Testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi Development: Targeted siRNA, qPCR Assays
- Liposome Encapsulation Services: siRNA, miRNA, DNA, Proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting: Technology Development and Evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING SERVICES
- RNA INTERFERENCE (RNAi) SERVICES
- In Vivo siRNA Delivery and Tissue Targeting (In Vivo RNAi)
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi Gene Knockdown Cell Lines
- RNAi Screen Pathway Analysis: siRNA and microRNA
- RNAi Manufacturing and CRO Services
- PCR Cloning: DNA Vector Construction (shRNA)
- Quantitation sh/siRNA-induced Knockdown by qRT-PCR and WB
- Genome-wide siRNA and microRNA High-throughput Screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA Liposome Encapsulation Service
- Transient (siRNA) and Stable (shRNA) Transfection Services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION SERVICES
- MICROORGANISM ID SERVICE
Stable RNAi Cell Line Development: shRNA Gene Knockdown
Stable RNAi Cell Line Generation: shRNA-induced stable gene knockdown
Gene silencing, also known as RNA interference (RNAi) is a biological phenomenon in which gene expression is silenced due to the specific degradation of the corresponding mRNA. This phenomenon occurs when double stranded RNA (dsRNA) in combination with RNA-inducible silencing complex (RISC) stimulates the sequence specific reduction of protein production of particular gene(s).
Altogen Labs offers development of inducible RNAi stable cell lines expressing shRNA constructs that target genes of interest. RNAi stable cell lines expressing shRNA constructs targeting genes of interest are important expression research tools for studying gene function in mammalian cells. However, generation of shRNA-expressing and tetracycline-inducible RNAi stable cancer cell lines can be very complex with all the required controls involved (e.g., efficiency of gene silencing, control for off-target effects, and promoter compatibility).
Altogen Labs can generate stable knockdown (RNAi) cell lines based on individual client requirements and project specifications. Our scientists offer years of extensive cell culture experience and cell line development expertise.
Standard RNAi stable cell line development services include:
a) Cloning -- plasmid DNAs encoding antibiotic-resistant genes, synthesis and cloning of shRNA constructs targeting genes of interest into plasmid DNAs, final plasmid DNA amplification, and maxi-prep purification, and
b) Stable cell line generation -- transfection of DNA plasmid into the cell line of choice, drug selection, generation of stable cells, validation of shRNA construct expression, and RNAi gene silencing by real-time RT-PCR (mRNA expression) and/or Western blot (protein expression).
Altogen Labs' life science research services include a wide range of pre-clinical studies, biology CRO services, xenograft and in vivo tox studies, custom liposome encapsulation, in vivo siRNA encapsulation and tissue-targeted delivery, as well as complete RNAi gene silencing services that include gene targeting, cloning of shRNA and microRNA into plasmid of choice, development of TET-inducible RNAi stable cell lines, siRNA synthesis and chemical modification, functional in vitro validation, and other gene silencing services.
Please send the details of your project for an accurate quote and timeline estimate (contact e-mail: info@altogenlabs.com or call Altogen Labs technical support at 512-433-6177)
see also: Generation of Stable Cell Line in 28 Days | Stable RNAi Cell Line Development: shRNA Gene Knockdown | Protein overexpression: Mammalian stable cell line development