Altogen Labs extensive biotechnology and pharmacology research services include the following:
- PRE-CLINICAL RESEARCH SERVICES
- Pharmacology and toxicology testing: IC50 for Tumor Cell Lines
- Xenograft Animal Service: Immunocompromised NOD/SCID Mice
- Therapeutic RNAi development: targeted siRNA, qPCR assays
- Liposome Encapsulation services: siRNA, miRNA, DNA, proteins
- ELISA and Cell-Based Assay Development (Cell Cycle, Viability)
- Apoptosis Assay: Caspase-3 and Caspase-7 Cell Line Screen
- RNAi Consulting Services: technology delevopment & evaluation
- GENERATION OF STABLE CELL LINES
- CELL BANKING
- RNA INTERFERENCE (RNAi) SERVICES
- In vivo siRNA delivery and tissue-targeting
- siRNA Transfection and RNAi Cell-based Library Screening
- Generation of Tet-inducible RNAi gene knockdown cell lines
- RNAi screen pathway analysis: siRNA and microRNA library
- RNAi manufacturing and CRO services
- PCR Cloning: shRNA-encoding vector construction
- In Vivo Stable siRNA and microRNA - Chemical modification
- Quantitation sh/siRNA-induced knockdown by qRT-PCR and WB
- Reporter gene assays and Stable RNAi cell line development
- Genome-wide siRNA and microRNA high-throughput screens
- Stable RNAi Cell Line Generation: Stable Gene Knockdown
- siRNA liposome encapsulation service
- Transient (siRNA) and stable (shRNA) transfection services
- BIOLOGY LABORATORY CRO SERVICES
- BIOREMEDIATION
- BACTERIA ID 16S SEQUENCING
Stable RNAi Cell Line Development: shRNA Gene Knockdown
Stable RNAi Cell Line Generation: shRNA-induced stable gene knockdown
Altogen Labs offers the development of inducible RNAi stable cell lines expressing shRNA constructs that target genes of interest. RNAi stable cell lines expressing shRNA constructs targeting genes of interest are important gene expression research tools, however development of tetracycline-inducible RNAi stable cancer cell lines can be very complex, time-cosuming and laborious to produce with all the necessary controls involved (e.g., efficiency of gene silencing, control for off-target effects, and promoter compatibility). Altogen Labs offers the generation of stable knockdown (RNAi) cell lines based on individual client requirements and project specifications. Company scientists bring years of extensive cell culture experience and cell line development expertise.
Standard RNAi stable cell line development service include:
a) cloning (plasmid DNAs encoding antibiotic-resistant genes, synthesis and cloning of shRNA constructs targeting genes of interest into plasmid DNAs, final plasmid DNA amplification, and Maxi-prep purification), and
b) stable cell line generation (transfection of plasmid DNA into the cell line of choice, drug selection, generation of stable cells, validation of shRNA construct expression, and RNAi gene silencing by real-time RT-PCR (mRNA expression) and/or Western Blot (protein expression).
Two clonal cell lines (one cryovial each) are delivered to the customer.
Altogen Labs provide innovative life science research services, including variety of pre-clinical studies, development of stable cell lines, in vivo siRNA delivery and complete A-to-Z RNAi gene silencing services: gene targeting, development of Tet-inducible RNAi stable cell lines, siRNA synthesis, chemical modification, functional in vitro validation, siRNA encapsulation, in vivo siRNA protection and tissue targeted delivery.
Please let us know the details of your project, so that we can provide you an accurate quote (contact e-mail: info@altogenlabs.com) and timeline estimate.
see also: Generation of Stable Cell Line in 28 Days | Stable RNAi Cell Line Development: shRNA Gene Knockdown | Protein overexpression: Mammalian stable cell line development