Altogen Labs CRO Services:
Stable RNAi Cell Line Generation: shRNA-induced stable gene knockdown
Gene silencing, also known as RNA interference (RNAi) is a biological phenomenon in which gene expression is silenced due to the specific degradation of the corresponding mRNA. This phenomenon occurs when double stranded RNA (dsRNA) in combination with RNA-inducible silencing complex (RISC) stimulates the sequence specific reduction of protein production of particular gene(s).
Altogen Labs offers development of inducible RNAi stable cell lines expressing shRNA constructs that target genes of interest. RNAi stable cell lines expressing shRNA constructs targeting genes of interest are important expression research tools for studying gene function in mammalian cells. However, generation of shRNA-expressing and tetracycline-inducible RNAi stable cancer cell lines can be very complex with all the required controls involved (e.g., efficiency of gene silencing, control for off-target effects, and promoter compatibility).
Altogen Labs can generate stable knockdown (RNAi) cell lines based on individual client requirements and project specifications. Our scientists offer years of extensive cell culture experience and cell line development expertise.
Standard RNAi stable cell line development services include:
a) Cloning -- plasmid DNAs encoding antibiotic-resistant genes, synthesis and cloning of shRNA constructs targeting genes of interest into plasmid DNAs, final plasmid DNA amplification, and maxi-prep purification, and
b) Stable cell line generation -- transfection of DNA plasmid into the cell line of choice, drug selection, generation of stable cells, validation of shRNA construct expression, and RNAi gene silencing by real-time RT-PCR (mRNA expression) and/or Western blot (protein expression).
Altogen Labs' life science research services include a wide range of pre-clinical studies, biology CRO services, xenograft and in vivo tox studies, custom liposome encapsulation, in vivo siRNA encapsulation and tissue-targeted delivery, as well as complete RNAi gene silencing services that include gene targeting, cloning of shRNA and microRNA into plasmid of choice, development of TET-inducible RNAi stable cell lines, siRNA synthesis and chemical modification, functional in vitro validation, and other gene silencing services.
Please send the details of your project for an accurate quote and timeline estimate (contact e-mail: email@example.com or call Altogen Labs technical support at 512-433-6177)